Descripción o resumen: The companion to this book, Protein Structure: A Practical Approach, gives methods of determining the structure of a newly discovered protein, but that is only part of the story. Protein Function: A Practical Approach completes the picture by providing the techniques for elucidating the functional properties of proteins, which can vary enormously, from simple structural roles to catalysis, from information transmission to energy control. This collection of tried-and-tested protocols will be applicable to many proteins, and can be performed in an average laboratory, without the need for specialized equipment. 1.: Identifying proteins by two-dimensional gel; electrophoresis 2.: Expressing cloned genes in Escherichia coli 3.: Folding proteins 4.: Ligand binding 5.: Ligand blotting 6.: Affinity labelling 7.: Chemical cross-linking and protein function 8.: Analysis of sequence-specific DNA-binding proteins 9.: Purification of sequence-specific DNA-binding proteins by affinity chromatography 10.: Chemical modification Readership: Postgraduate and post-doctoral researchers and academic staff in the general area of protein science
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Autor de la reseña
Subido por: Ignacia Pérez López
Fecha: 27/5/2018
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